Generator

Part:BBa_K1073015

Designed by: iGEM Team Braunschweig 2013   Group: iGEM13_Braunschweig   (2013-09-17)

Constitutively expressed transcription regulator LuxR + LVA

This device consists of a strong constitutive promoter (BBa_J23100) combined with RBS (BBa_B0032) and the coding region of the transcription regulator LuxR (BBa_C0062). This results in a constitutively high transcription level of LuxR.


Usage and Biology

In combination with N-3-oxohexanoyl homoserine lactone (N-3-oxohexanoyl-HSL) synthesized by LuxI, LuxR is able to activate the inducible promoter of the lux quorum sensing system of Vibrio fischeri.

LuxR binds to the regulatory lux promoter region and in the presence of N-3-oxohexanoyl-HSL it activates gene expression (Fuqua et al., 1994).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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